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Люстра потолочная светодиодная Stilfort 2079/03/06C серия Blot

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2079 | Sigma-Aldrich

western blot (4) Clonality. monoclonal (2) polyclonal (2) Host Species. mouse (2) rabbit (2) synthetic (2) Species Reactivity. human (4) mouse (2) rat (3) Biological Source. Mouse (2) Rabbit (2) Others (2) Recombinant Host. E. coli (1) Featured Industry. Agriculture (2) Cleaning Products (2) Cosmetics (2) Diagnostic Assay Manufacturing (1) Environmental (1) Food and Beverages (2) Personal Care ...

Criterion™ Blotter | Life Science Research | Bio-Rad

Mini Trans-Blot Cell: Criterion Blotter: Trans-Blot Cell: Trans-Blot Plus Cell: Semi-Dry Transfer Trans-Blot SD Cell: Blotting area (W x L) 10 x 7.5 cm: 15 x 9.4 cm: 16 x 20 cm: 28 x 26.5 cm: 24 x 16 cm: Transfer Parameters: Number of cassettes: 2: 2: 1 for high-intensity transfer 2 with cooling 3 without cooling: 3-Buffer requirement: 450 ml ...

Anti-mouse IgG, HRP-linked Antibody | Cell Signaling ...

Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times. Limited Uses. Except as otherwise ...

Clean-Blot™ IP Detection Reagent (HRP)

The Clean-Blot IP Reagent allows trouble-free Western blot detection of target proteins following IP assays (immunoprecipitation). It works by specifically binding to functional primary antibodies (whole IgG) without also binding to fragments of the IP antibodies, which usually accompany the immunoprecipitated protein through electrophoresis and membrane transfer. The Clean-Blot IP Reagent and ...

Increased processing of SINE B2 ncRNAs unveils a novel ...

After incubating primary antibody, blots were washed using 0.1% PBST and reprobed with anti-rabbit-HRP conjugate secondary antibody (1:5000; abcam; ab97051). Proteins were visualized using Pierce enhanced chemiluminescence detection system (Thermo Fisher Scientific; 32106). Blots were imaged in an AI600 imager (GE Healthcare) and densitometry ...

The RNA polymerase II subunit RPB‐9 recruits the ...

Western blot quantification of RPB‐9 expression in wild‐type, rpb‐9 (mj261), and rpb‐9 rescue animals. rpb‐9 alleles gk508135 and gk357431 (Thompson et al, 2013) are additional controls that do not show piRNA sensor desilencing. RPB‐9 domain conservations.

NFkB p65 (Transcription Factor p65, Nuclear Factor NF ...

Recommended Dilution: Western Blot: 1-4ug/ml Immunohistochemistry: Paraffin Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior ...

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TRIT1 Antibody (NBP2-20727): Novus Biologicals

Western Blot 1:500-1:3000; Immunohistochemistry 1:100-1:1000; Immunohistochemistry-Paraffin 1:100-1:1000; Theoretical MW: 53 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Packaging, Storage & Formulations ...

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Naphthoquinone Derivative PPE8 Induces Endoplasmic ...

Stress markers p-IRE1, GRP78, and p-JNK were examined by western blot, ER was confirmed by ER-specific dye immunostain, and fluorescent area was analyzed by ImageJ software. Whether p53 was involved in PPE8-induced ER stress was investigated by p53 siRNA knockdown in A549 cells. Figure 1 (a) Structures of PPE8, (b) A549, and H1299 cells were incubated without or with indicated concentrations ...

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Collective cell durotaxis emerges from long-range ...

Western blot analysis showed that α-catenin levels on the day of the experiment were 57 ± 13% of those observed in control cells. ... J. 84, 2071 – 2079 (2003). doi: 10.1016/S0006-3495(03)75014-0 pmid: 12609908. OpenUrl CrossRef PubMed Web of Science. M. Dembo, Y. L. Wang, Stresses at the cell-to-substrate interface during locomotion of fibroblasts. Biophys. J. 76, 2307 – 2316 (1999 ...

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Coronary Serum Exosomes Derived from Patients with ...

2079 Thheerraannoossttiiccss 2018; 8(8): 2079-2093. doi: 10.7150/thno.21895 Research Paper Coronary Serum Exosomes Derived from Patients with Myocardial Ischemia Regulate Angiogenesis through the miR-939-mediated Nitric Oxide Signaling Pathway Hao Li1*, Yiteng Liao1*, Lei Gao2, Tao Zhuang2, Zheyong Huang3, Hongming Zhu2 , Junbo Ge1,3 1. Department of Cardiology, Shanghai Tenth People’s ...

Mammaglobin 1 antibody Anti-Mammaglobin 1|ELISA WB

Mouse monoclonal Mammaglobin 1 antibody. Home. Primary Antibodies . View All Primary Antibodies ; Monoclonal Antibodies

MicroRNA induction by copy number gain is associated with ...

Introduction. Copy number alternations, such as genomic amplifications and deletions, induce oncogene expression and repress tumor suppressor genes 1.Even low-level copy number gains have been found to contribute to oncogene induction and have been associated with malignant phenotypes.

Disparity of microaneurysm count between ultrawide field ...

MAs on CI were defined as a punctate red lesion with sharp margins approximately 20–125 μm in diameter (the diameter of a retinal vein on the margin of the optic disc).6 20 The definition of MA excluded flame-shaped haemorrhages and large blot haemorrhages. On FA, MA diameters of approximately 20–125 µm were used. The lower limit of 20 µm on FA is similar to previous studies given the ...

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p21 Antibody (MA5-14949)

Western blot analysis of p21 Waf1/Cip1 was performed by loading HUVEC and HeLa cell lysates on a SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% nonfat dry milk in TBST for 1 hour at room temperature. The membrane was probed with a p21 Waf1/Cip1 monoclonal antibody (Product # MA5-14949) at a dilution of 1:1000 overnight at 4C, followed by an anti-rabbit ...

Blotting Membranes - Bio-Rad Laboratories

western blot preparation. For more information, contact your local Bio-Rad representative, visit us on the Web at discover.bio-rad.com, or call toll free at 1-800-4BIORAD (1-800-424-6723). Supports That Mean Results PVDF Membranes Ideal for: Protein blotting (Immun-Blot® PVDF membrane) ™(Sequi-Blot PVDF membrane)

Failures of nerve regeneration caused by aging or chronic ...

Blots were analyzed and densitometric quantification was performed using Bio-Rad Imagelab. Protein levels were determined by normalizing the protein of interest against the house keeping protein (GAPDH or calnexin). All blots were then normalized to one sample (e.g. 1 week after injury, control cells) to account for any difference between each blot. Each experiment was performed at least three ...

Alterations in Glucose Metabolism Due to Decreased ...

(Merck Millipore, MA, USA, 03-100 at 1:10,000) or mouse anti- -actin antibody (Santa Cruz Biotechnology, Inc., Dallas, ... BloT Quant HRP or Ultra Sensitive HRP Substrate (Takara Bio, Inc., Shiga, Japan) and Amersham hyperfilm ECL (GE Healthcare Ltd., Buckinghamshire, UK). 2.6. Cell Proliferation Assay After the cells were cultured for 24 to 60 h, a volume of 10 L/well of the Cell Counting ...

GADD34 is a modulator of autophagy during starvation ...

For Western blot analysis, 20 μg of protein fraction samples was boiled and loaded onto a precast NuPAGE 4–12% bis-tris protein gel (Thermo Fisher Scientific, catalog number NP0321BOX) and run in NuPAGE MES SDS Running Buffer (Thermo Fisher Scientific, catalog number NP0002). After electrophoresis, proteins were transferred to a nitrocellulose membrane using the iBlot 2 Gel Transfer Device ...

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Biology | Free Full-Text | Alterations in Glucose ...

After washing with PBS containing 0.05% Tween 20 (PBS-T), the membranes were incubated with a mouse anti-HNRNPM antibody (Merck Millipore, MA, USA, 03-100 at 1:10,000) or mouse anti-β-actin antibody (Santa Cruz Biotechnology, Inc., Dallas, TX, sc-47778 at 1:12,000) diluted in Can Get Signal ® Solution 1 (Toyobo

A Whole-Cell Biosensor for Point-of-Care Detection of ...

Western Blot Analysis. Proteins extracted from 1 × 10 9 cells were resolved by 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the gel was transferred to a polyvinylidene difluoride membrane. The membrane was blocked in 5% nonfat milk at room temperature for 1 h, followed by incubation with anti-His (GenScript, 6G2A9, 1:2000) or anti-GAPDH (Abcam, GA1R, 1:5000) primary ...

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Secondary Antibodies - Jackson ImmunoResearch

Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative: None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.)

Myc-Tag (71D10) Rabbit mAb | Cell Signaling Technology

Western blot analysis of extracts from untransfected control cells (-) and transfected cells overexpressing Myc-Bcl-2 (+), using Bcl-2 Antibody #2872 (left) and Myc-Tag (71D10) Rabbit mAb (right). Confocal immunofluorescent analysis of 293 cells stably expressing Myc-tagged ADORA2A (left) versus wild-type 293 cells (right) using Myc-Tag (71D10) Rabbit mAb (green). Blue = DAPI #4083 ...

Search Results | Sigma-Aldrich

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Immunoprecipitation / Western Blots - SCBT

Immunoprecipitation / Western Blots. Prepare a total cell lysate as described under Western blot procedure in protocol 1. Preclear whole cell lysate (optional step) as follows: To approximately 1 ml of whole cell lysate or tissue extract (see Whole Cell Lysates or Tissue Extract Tables), add 0.25 µg of the appropriate control IgG (corresponding to the host species of the primary antibody ...

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Blot - Eurospital - Divisione Diagnostica

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PI4KIIIβ is a therapeutic target in chromosome 1q ...

Cancer cells produce and secrete a variety of proteins that promote tumorigenesis, in a process known as malignant secretion. Tan et al. identified a region of chromosome 1 that is often amplified in cancer and plays a key role in malignant secretion. In particular, the authors discovered a mechanism by which a Golgi-associated enzyme called PI4KIIIβ promotes secretion of proteins involved in ...

Secondary Antibodies - Jackson ImmunoResearch

Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Preservative: None Suggested Working Concentration or Dilution Range: 10-20 µg / ml Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density ...

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Thermistors - Newicshop

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Guest post: How ‘urban heat islands’ will intensify ...

We used this dataset in our new paper to estimate summer UHI intensity changes in the 10 most populous cities in England between 1981 and 2079. These cities are represented by increased urban fractions, i.e. the fraction of urban land use in each model grid cell, in the UKCP18 regional model.

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Thermistors - Newicshop

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